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Invitrogen™ NOTCH-1 Human ELISA Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Sandwich ELISA Kit

$494.00

Specifications

Storage Requirements 2°C to 8°C
Assay Sensitivity 20 pg/mL
Assay Range 28.67-7000 pg/mL
Test Time 1 hr. 20 min.
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
EHNOTCH1 Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.
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Invitrogen™
EHNOTCH1
96 Tests
Pack for $494.00
Add to cart
 
Description

Description

The Human NOTCH-1 ELISA quantitates Hu NOTCH-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NOTCH-1. Principle of the method The Human NOTCH-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

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Specifications

Specifications

2°C to 8°C
28.67-7000 pg/mL
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
<10%
20 pg/mL
1 hr. 20 min.
<12%
SDS
Product Certifications
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