NOTCH 1 Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 100401407S

Catalog No. 50-105-7414

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Specifications

• Antigen: NOTCH 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Notch1
• Gene Alias: 9930111A19Rik; AOS5; AOVD1; BOS_12152; Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); hN1; lin-12; major type A protein; Mis6; Motch; Motch A; mT14; N1; Neurogenic locus notch homolog protein 1; NEXT; NICD; NOTCH; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; NOTCH1; p300; RP23-306D20.12; RP23-306D20.12-001; TAN1; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
• Host Species: Rabbit
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody

• Applications: Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 90 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.1% sodium azide; pH 7.2
• Gene Accession No.: P46531, Q01705, Q07008
• Gene Symbols: Notch1
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
• Regulatory Status: RUO
• Gene ID (Entrez): 18128, 25496, 4851
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid