NOTCH 2Rabbit anti-Canine, Chimpanzee, Human, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 100401406

Catalog No. 50-199-0069

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.

Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved Notch signaling pathway protein. NOTCH2 is a type 1 transmembrane protein with an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. NOTCH2 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.

Specifications

• Antigen: NOTCH 2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOTCH2
• Gene Alias: AGS2; AI853703; HJCYS; hN2; Motch B; N2; N2ECD; N2ICD; Neurogenic locus notch homolog protein 2; Notch 2; Notch 2 extracellular truncation; Notch 2 intracellular domain; notch gene homolog 2; Notch homolog 2; Notch2
• Host Species: Rabbit
• Quantity: 200 μL
• Primary or Secondary: Primary
• Target Species: Canine, Chimpanzee, Human
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 75 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.1% sodium azide; pH 7.2
• Gene Accession No.: Q04721
• Gene Symbols: NOTCH2
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Regulatory Status: RUO
• Gene ID (Entrez): 457173, 483148, 4853
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid