Invitrogen™ NOTCH1 Monoclonal Antibody (mN1A), FITC

Catalog No.	Quantity
PIMA516862	100 μg

Catalog No. PIMA516862 Supplier Invitrogen™ Supplier No. MA516862

Description

Mouse Monoclonal Antibody

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Mouse Notch 1 antibody, clone mN1A recognizes Notch 1, one of the four major transmembrane receptors (Notch 1-4) of the Notch signaling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands.

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

Specifications

• Antigen: NOTCH1
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: mN1A
• Concentration: 0.1 mg/mL
• Conjugate: FITC
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Gene: Notch1
• Gene Accession No.: P46531, Q01705
• Gene Alias: 9930111A19Rik; AOS5; AOVD1; Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); hN1; lin-12; major type A protein; Mis6; Motch; Motch A; mT14; N1; neurogenic locus notch homolog protein 1; NEXT; NICD; NOTCH; notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; notch receptor 1; Notch1; p300; RP23-306D20.12; RP23-306D20.12-001; Tan1; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
• Gene Symbols: Notch1
• Host Species: Mouse
• Immunogen: Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18128, 4851
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1