Invitrogen™ NOTCH3 Monoclonal Antibody (HMN3-133), APC, eBioscience™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3138	Invitrogen™ 17576382	100 μg

Description

Description: This HMN3-133 monoclonal antibody reacts with mouse Notch3, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription. Notch 3 expression has been demonstrated on regulatory T cells and some thymocyte subsets, including CD4-CD8- and CD8SP cells. In addition, this Notch receptor is prevalent in vascular smooth muscle and the central nervous system. In addition to its role in stem cell hematopoiesis, Notch 3 plays a pivotal role in mammalian T cell lineage commitment and thymocyte development. Specifically, Notch 3 has been implicated in the CD4-CD8- double negative (DN) to CD4+CD8+ double positive transition with greatest expression in DN3 (CD4-CD8-CD25+CD44-) cells. Notch 3 may also be involved in regulating the pre-TCR checkpoint via crosstalk with the NFkappaB and E2A signaling pathways. Finally, overexpression of Not...

NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Specifications

• Antigen: NOTCH3
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Armenian Hamster
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q61982
• Gene ID (Entrez): 18131
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: HMN3-133
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Mouse
• Gene: Notch3
• Gene Alias: AW229011; CADASIL; CADASIL1; CASIL; hpbk; IMF2; LMNS; N3; Neurogenic locus notch homolog protein 3; notch 3; Notch 3 extracellular truncation; Notch 3 intracellular domain; Notch gene homolog 3; Notch homolog 3; NOTCH3
• Gene Symbols: Notch3
• Product Type: Antibody