Invitrogen™ NOTUM Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595811

Catalog No. PIPA595811

Description

Immunogen sequence: SEGRKTWRRR GQQPPPPPRT EAAPAAGQPV ESFPLDFTAV EGNMDSFMAQ VKSLAQSLYP CSAQQLNEDL RLHLLLNTSV TCNDGSPAGY YLKESRGSRR WLLFLEGGWY CFNRENCDSR YDTMRRLMSS RDWPRTRTGT GILSSQPEEN PYWWNANMVF IPYCSSDVWS GASSKSEKNE YAFMGALIIQ EVVRELLGRG LSGAKVLLLA GSSAGGTGVL LNVDRVAEQL EKLGYPAIQV RGLADSGWFL DNKQYRHTDC VDTITCAPTE AIRRGIRYWN GVVPERCRRQ FQEGEEWNCF F; Positive Samples: BxPC-3, HepG2.

Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the a/beta-hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.

Specifications

• Antigen: NOTUM
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOTUM
• Gene Alias: [Wnt protein] O-palmitoleoyl-L-serine hydrolase; 5730593N15Rik; hNOTUM; NOTUM; notum pectinacetylesterase homolog; notum pectinacetylesterase homolog (Drosophila); NOTUM, palmitoleoyl-protein carboxylesterase; OK/SW-CL.30; Palmitoleoyl-protein carboxylesterase NOTUM; protein notum homolog; RGD1307119
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 147111
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.23 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q6P988
• Gene Symbols: NOTUM
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human NOTUM (NP_8485883).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov