EMD Millipore™ Novagen™ B834pLysS Competent Cells

For production of protein from target genes cloned in pET vectors by induction with IPTG

Manufacturer: EMD Millipore™ 690423

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Catalog No. 69-042-3

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    Quantity 0.4mL
    Type B834pLysS Competent Cells
    Storage Requirements ≤ -70°C

    • B834 is parental strain for BL21 (1)
    • Protease-deficient hosts are methionine auxotrophs
    • Allows high specific-activity labeling of target proteins with methionine and selenomethionine for crystallography
    • pLysS strains express T7 lysozyme
    • Suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability

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