Invitrogen™ Human IL-4 ELISA Kit

Description

ELISA

The Human Interleukin 4 (Hu IL-4) ELISA quantitates Hu IL-4 in human serum. The assay will exclusively recognize both natural and recombinant Hu IL-4. Principle of the method The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Accession Number: P05112
• Assay Range: 14.5 to 1695 pg/mL
• Assay Sensitivity: 1.2 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Serum
• Target Species: Human
• For Use With (Application): Colorimetric
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3565
• Gene Symbol: BCGF-1,BCGF1,BSF-1,BSF1,IL-4
• Interassay CV: 5.1%
• Intraassay CV: 3.8
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Incubation Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls
• Label or Dye: HRP
• Precision: 2 pg/mL
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Serum 100 μL
• Storage Requirements: 2°C to 8°C
• Target: IL-4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins

Safety and Handling

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