Invitrogen™ MMP3 Human Matched Antibody Pair

Matched Antibody Pair

Supplier:  Invitrogen™ CHC1543

Catalog No. CHC1543

Includes: Antibody Pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.

Description

The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.

Order Info

Shipping Condition: Wet Ice

Specifications

• Conjugate: HRP
• Gene Alias: CHDS6, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1
• Quantity: 10 x 96 Tests
• Gene ID (Entrez): 4314
• Content And Storage: 2°C to 8°C
• For Use With (Equipment): Colorimetric Microplate Reader

• Gene Accession No.: P08254
• Gene Symbols: MMP3
• Regulatory Status: RUO
• Includes: Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
• Product Type: ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.