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Invitrogen™ Novex™ pH 3-10 IEF Protein Gels, 1.0 mm, 10-well Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Separation Range: pH 3 to 10; Separation Type: Native; For Use With: Mini Gel Tank, XCell SureLock Mini

Manufacturer:  Invitrogen™ EC6655BOX

Catalog No. EC6655BOX


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Includes: One box of 5 gels

Description

Description

Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

  • IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels
  • Pre-focusing is not required
  • Total run time is approximately 2.5 hours
  • Made of 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes; They do not contain denaturing reagents

Sample Loading Volume: 25µL; 1D Well

Isoelectric Focusing, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

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Order Info

Shipping Condition: Wet Ice

Specifications

Specifications

pI (Charge)
25 µL
Novex®
1D Well
8 cm
8 cm
Mini
5%
Isoelectric Focusing
Mini Gel Tank, XCell SureLock Mini
10-well
One box contains 5 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life up to 6 months.
Novex™
Wet Ice
1.0 mm
13.5 cm
8 cm
Homogeneous Page
pH 3 to 10
Ampholyte
1 mm
Protein
5/Pk.
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For Research Use Only. Not for use in diagnostic procedures.