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Invitrogen™ Human p38 MAPK (Total) ELISA Kit

Catalog No. KHO0061
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Quantity:
96 Tests

ELISA

The Human p38 MAPK (Total) ELISA quantitates Hu p38 MAPK (Total) in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu p38 MAPK (Total). Principle of the method The Human p38 MAPK (Total) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q16539
Assay Range 31.2 to 2000 pg/mL
Assay Sensitivity <16 pg/mL
Concentration 31.2 to 2000 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Substrate Type Serine/Threonine Kinases
Target Species Mouse, Rat
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1432
Gene Symbol CSBP,CSBP1,CSBP2,CSPB1,EXIP,Mxi2,PRKM14,PRKM15,RK,SAPK2A,p38,p38ALPHA
Incubation Time 4 hr.
Interassay CV 7.3%
Intraassay CV 5.1
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene p38
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 10 μL
Storage Requirements 2°C to 8°C
Target p38 MAPK alpha
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs
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