Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Tau (Total) Human ELISA Kit
GSA_VA

Designed to detect and quantify the level of human Tau protein, regardless of phosphorylation state

$636.00 - $1068.00

Specifications

Concentration 31 to 2000pg/mL
Conjugate Biotin
Gene ID (Entrez) 4137
Marker Gene Tau
Sample Type Cerebrospinal Fluid, Supernatant
View More Specs

Includes: Pre-coated plate, standard, biotinylated detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
KHB0042
GSA_VA
Encompass
View Documents
Invitrogen™
KHB0042
2 x 96 Tests
Each for $1,068.00
Add to cart
 
KHB0041
Encompass
View Documents
Invitrogen™
KHB0041
96 Tests
Each for $636.00
Add to cart
 
Description

Description

The Human Tau (Total) ELISA quantitates Hu Tau in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Tau, independent of phosphorylation state. Principle of the method The Human Tau solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The Tau (Total) Human ELISA Kit is intended for detection of Tau from cerebral spinal fluid (CSF) or from tissue culture supernatant using 96-well plates and a microplate reader.

Easy-to-run sandwich ELISA
A monoclonal capture antibody specific for Hu Tau has been coated onto the wells of the 96-well plate provided. During the first incubation, standards of known Tau content and unknown samples are pipetted into the wells, and the Tau antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for Tau is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Tau protein captured during the first incubation. After washing, a horseradish peroxidase-labeled, anti-rabbit IgG is added. This binds to the detection antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu Tau present in the original specimen and the optical density can be read on a standard microplate reader.

Convenient kit format
The kit comes with a capture antibody precoated onto the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips, allowing you to run as few samples as you want. Standards provided in the kit can be used to obtain quantitative results from a standard curve or as a positive control.

  • Easy to run
  • Convenient precoated, removable 8-well strips
  • Validated kits for accuracy and consistency
  • Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

See protocol insert for more information on validation.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping condition: Wet ice

Specifications

Specifications

31 to 2000pg/mL
4137
Cerebrospinal Fluid, Supernatant
<10 pg/mL
Biotin
Tau
Cerebrospinal Fluid,50 μL; Supernatant, 50 μL
Human
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit