Invitrogen™ Human Prostaglandin E2 ELISA Kit

Description

ELISA

The Human Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples. Principle of the method This assay is based on the competition between Prostaglandin E 2 (PGE2) and a PGE2 alkaline phosphatase tracer for a limited amount of PGE2 specific monoclonal antibody. Because of the competition between PGE2 in the sample and PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Prostaglandin E2 (PGE2) is a lipid mediator derived from arachidonic acid through the actions of cyclooxygenase (COX) enzymes and terminal prostaglandin E synthases (PGES). It plays a significant role in mediating inflammation, pain sensation, and fever by acting on four receptor subtypes: EP1, EP2, EP3, and EP4. PGE2's involvement in inflammatory responses is well-documented, particularly in diseases such as rheumatoid arthritis and osteoarthritis. Various medications, including nonsteroidal anti-inflammatory drugs (NSAIDs) and selective COX-2 inhibitors, are used to reduce PGE2 production, thereby alleviating inflammation and pain. However, these drugs can have side effects, such as gastrointestinal issues and increased risk of thrombosis. Additionally, PGE2 has a critical role in immune regulation, affecting T cell function and, by extension, the adaptive immune response. It also has a significant impact on skeletal disorders and pain through mechanisms that are still being explored.

Specifications

• Accession Number: NM
• Assay Range: 31.3 to 4000 pg/mL
• Conjugate: AP
• Product Type: ELISA
• Sample Type: Plasma, Supernatant, Urine
• Target Species: Human
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Incubation Time: 3 hr.
• Interassay CV: 11.7%
• Intraassay CV: 6
• Kit Contents: Pre-coated 96 well plate, Standard, Tris Buffer, Alkaline Phosphatase Tracer, Leukotriene B4 Antiserum, DEA Buffer, Wash Buffer, pNPP tablets, Adhesive Plate Covers
• Label or Dye: AP
• Marker Gene: Prostaglandin E2
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Supernatant 100 μL, Urine 100 μL
• Storage Requirements: Store kit at -20°C. Refer to product documentation for component specific storage temperature.
• Target: PGE2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov