Invitrogen™ SAA Livestock ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ KAA0021

Catalog No. KAA0021

Includes: SAA Antibody-Coated Wells, 1 x 96 well plate
Wash Buffer Concentrate, 1 x 50mL
Sample/Calibrator Diluent Concentrate, 1 x 25mL
SAA Calibrator, 1 lyophilized vial
Anti-SAA-HRP Conjugate, 1 x 6mL
Stabilized Chromogen, Tetramethylbenzidine (TMB), 1 x 11mL
Stop Reagent, 1 x 11mL
Detailed Protocol, 1 protocol

Description

The Livestock SAA ELISA Kit quantifies equine, porcine, feline, bovine and canine Serum Amyloid A (SAA) in serum and plasma. The assay will recognize both the natural and recombinant SAA. Principle of the method The Livestock SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Serum amyloid A (SAA) proteins comprise a family of small (12-14 kDa, 104-112 amino acid residues), differentially expressed proteins that are highly conserved among vertebrates. SAA proteins are involved in the acute phase responses; these are the immediate early host responses to inflammation. During the acute phase, circulating SAA levels are increased by 100-1000 fold, reaching concentrations of up to one milligram per milliliter. Liver is the major site of SAA synthesis, although extrahepatic expression has also been reported. Mouse SAA gene family maps to chromosome 7. In mouse, five SAA genes and four protein products have been identified: mouse SAA1, SAA2, SAA3 are the acute phase isoforms, SAA5 is constitutively expressed, and SAA4 is a pseudogene. SAAs belong to a category of acute phase proteins that also includes C-reactive protein (CRP), haptoglobin, ceruloplasmin, complement components C3 and C4, a1-acid glycoprotein, a1-proteinase inhibitor, and fibrinogen.

Specifications

• Accession Number: P19708,P19857,P35541
• Assay Sensitivity: 1.5μ g/mL
• Product Type: ELISA Kit
• Target Species: Bovine, Canine, Equine, Porcine
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: PIG4,SAA,SAA2,TP53I4
• Interassay CV: 11.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution
• Marker Gene: SAA
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 9.4-150μg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 100034017, 100525856, 616035, 751814
• Incubation Time: 1.25 hr.
• Intraassay CV: 5.9%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 1 μL; Serum, 1 μL
• Target: Bovine, Canine, Equine, Porcine
• Total Assay Time: 1 hr. 15 min.

For Research Use Only. Not for use in diagnostic procedures.