Novus Biologicals™ CHO K1 Whole Cell Lysate

Description

The CHO K1 Whole Cell Lysate is derived from Chinese hamster. It has been validated for the following applications: Western Blot, SDS_PAGE

Specifications

• For Use With (Application): Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
• Tissue: Reproductive
• Quantity: 0.5 mL
• Storage Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) 10% (v/v) Glycerol
• Purity: Multi-step
• Test Specificity: Cell Line: Chinese Hamster Ovary (CHO)
• Cellular Fraction: Whole
• Content And Storage: Store at -70°C. Avoid freeze-thaw cycles.

For Research Use Only