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Description
The MEF Whole Cell Lysate is derived from mouse. It has been validated for the following applications: Western Blot, SDS-PAGE
Specifications
Specifications
| For Use With (Application) | Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel. |
| Tissue | Bone/Cartilage |
| Storage Buffer | 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol |
| Purity | Multi-step |
| Test Specificity | MEF (Mouse Embryonic Fibroblast) whole cell extract lysate |
| Cellular Fraction | Whole |
| Content And Storage | Store at -70°C. Avoid freeze-thaw cycles. |
For Research Use Only
Product Title
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