Novus Biologicals™ Citrate Buffer Solutions

Description

For recovery of antigens masked by fixation in cross-linking fixatives, such as formalin

• 10X Citrate buffer for Immunohistochemistry
• Dilute one part buffer with nine parts distilled water
• Unit size: 500mL

Recommended Storage

Store at room temperature

Specifications

• pH: 6
• Quantity: 500 mL
• Content And Storage: Room Temperature
• For Use With (Application): The Antigen Retrieval Protocol is Recommended for Use in Tissues That Have Been Fixed in Formalin Only. Ensure That The Fixed Sections Are Adequately Embedded in Paraffin. Cut Tissue Sections to 4-5 Microns. Preparation of Working Solutions 1. The 10 x Concentrated Format Should be Diluted Tenfold with Distilled or Deionized Water. 2. Mix One Part of Concentrated Antigen Retrieval Solution with Nine Parts of Deionized or Distilled Water. 3. Shake The Bottle Vigorously to Completely Mix The Components of The Concentrate (The Solution May Separate Into Phases Over Time). 4. Store with Cap Tightly Secured. Protocol Recommendations 1. Deparaffinize and Rehydrate Tissue Sections. 2. Place Slides Into 1 x Retrieval Solution in a Slide Container (e.g. Coplin Jar, Tissue-Tek, Staining Dish or Metal Slide Canister). 3. Retrieve Sections Under Pressure 4. After Take-Off Reagent Jar Containing Slides From Pressure Cooker, Allow The Slides to Cool for 20 Minutes to Reach Room Temperature. 5. Wash Slides in Deionized Water and Then with Wash Buffer. Proceed with Immunostaining Recommendations in The Antibody Datasheet. 6. Gently Rinse by Gradually Adding DI Water to The Solution, Then Remove Slides and Rinse with DI Water.
• Buffer: Citrate Buffer
• Host Species: Human
• Product Type: 10X Citrate Buffer