Invitrogen™ NOX2 Monoclonal Antibody (54.1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA518052

Catalog No. PIMA518052

Description

This antibody is specific for NOX2/gp91phox (aa 382-389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100). Western blot results show a band at 65 kDa, which matches the predicted molecular weight. For IHC applications, perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining.

Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.

Specifications

• Antigen: NOX2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P04839
• Gene Symbols: Cybb
• Immunogen: Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 54.1
• Conjugate: Unconjugated
• Gene: Cybb
• Gene Alias: AMCBX2; C88302; Cgd; CGD91-phox; Cybb; Cyd; cytochrome b(558) subunit beta; cytochrome b-245 beta chain; cytochrome b-245 beta polypeptide; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; cytochrome b558 subunit beta; endothelial type gp91-phox; GP91 PHOX; GP911; GP91-1; GP91PHOX; gp91-phox; Heme-binding membrane glycoprotein gp91phox; IMD34; NADPH oxidase 2; neutrophil cytochrome b 91 kDa polypeptide; Nox2; p22 phagocyte B-cytochrome; p91PHOX; p91-PHOX; superoxide-generating NADPH oxidase heavy chain subunit
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 1536
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov