MilliporeSigma™ NOX4, Mouse, Unlabeled, Clone: 10A8.1,

Catalog No.	Quantity	Conjugate
MABC616MI	100 μL	Unconjugated

Catalog No. MABC616MI Supplier MilliporeSigma™ Supplier No. MABC616

Description

Mouse Monoclonal Antibody

NADPH oxidase 4 (UniProt Q9NPH5; also known as Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, Renal NAD(P)H-oxidase) is encoded by the NOX4 (also known as RENOX) gene (Gene ID 50507) in human. NOX4 belongs to the family of NADPH oxidases that modulate redox-dependent cellular signalling pathways via intracellular reactive oxygen species (ROS) generation. Seven family members have been identified in mammals (NOX1–5 and DUOX1/2) with member-specific cellular distribution, intracellular location, mechanisms of action and types of ROS produced. NOX4 produces predominantly H2O2, and is widely expressed in many cell types and adult tissues, including kidney, liver, heart and aorta. NOX4 plays an important role in homocysteine (Hcy) metabolism, which in turns is a determining factor that impacts on defence against environmental toxins. Nox-knocout in mice results in significantly decreased total plasma Hcy levels, causing markedly increased susceptibility to acetaminophen- (APAP-, paracetamol-) induced hepatoxicity as a result of significantly decreased cysteine production and hepatic GSH stores. Human NOX4 is a six-transmembrane (a.a. 17-37, 63-83, 104-124, 155-175, 189-209, 425-445) protein, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-16 and 446-578).

Specifications

• Antigen: NOX4
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 10A8.1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Mouse monoclonal IgG2bκ hybridoma culture supernatant without preservatives.
• Gene: NOX4, RENOX
• Gene Accession No.: Q9NPH5
• Host Species: Mouse
• Immunogen: GST-tagged recombinant protein corresponding to a fragment from the third extracellular loop of human NOX4.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Apoptosis & Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001137308
• Test Specificity: Immunogen construct region is present in human NOX4 spliced isoform 1, 2 (Nox4A), and 8, missing in isoform 5 (Nox4C), 7, and 9, partially truncated in isoform 3 (Nox4E), 4 (Nox4D), and 6 (Nox4B) reported by UniProt (Q9NPH5).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Culture Supernatant
• Isotype: IgG2b κ

For Research Use Only. Not for use in diagnostic procedures.