Invitrogen™ NPHP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA561148

Catalog No. PIPA561148

Description

Immunogen sequence: TVEDTNGSET GFRAWNVQSR GRIFLVSKPV LQINTVDVLT TMGAIPAGFR PSTLSQLLEE GNQFRANYFL QPELMPSQLA FRDLMWDATE GTIRSRPS Highest antigen sequence identity to the following orthologs: Mouse - 59%, Rat - 57%.

Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.

Specifications

• Antigen: NPHP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NPHP1
• Gene Alias: JBTS4; juvenile nephronophthisis 1 protein; nephrocystin 1; Nephrocystin1; nephrocystin-1; nephronophthisis 1 (juvenile); nephronophthisis 1 (juvenile) homolog; nephronophthisis 1 (juvenile) homolog (human); NPH1; NPHP1; SLSN1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4867
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O15259
• Gene Symbols: NPHP1
• Immunogen: Recombinant protein corresponding to Human NPHP1. Recombinant protein control fragment (Product #RP-96114).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG