Invitrogen™ NPHP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA593140

Catalog No. PIPA593140

Description

Immunogen sequence: PDLGILFELG ISYIRNSTGE RGELSCGWVF LKLFDASGVP IPAKTYELFL NGGTPYEKGI EVDPSISRRA HGSVFYQIMT MRRQPQLLVK LRSLNRRSRN VLSLLPETLI GNMCSIHLLI FYRQILGDVL LKDRMSLQST DLISHPMLAT FPMLLEQPDV MDALRSSWAG KESTLKRSEK RDKEFLKSTF LLVYHDCVLP LLHSTRLPPF RWAEEETETA RWKVITDFLK QNQENQGALQ ALLSPDGVHE PFDLSEQTYD FLGEMRKNAV; Positive Samples: Mouse lung, Rat brain; Cellular Location: Cell junction, Cell projection, Cytoplasm, adherens junction, cilium, cilium axoneme, cytoskeleton, tight junction.

Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.

Specifications

• Antigen: NPHP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NPHP1
• Gene Alias: JBTS4; juvenile nephronophthisis 1 protein; nephrocystin 1; Nephrocystin1; nephrocystin-1; nephronophthisis 1 (juvenile); nephronophthisis 1 (juvenile) homolog; nephronophthisis 1 (juvenile) homolog (human); NPH1; NPHP1; SLSN1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 296136, 4867, 53885
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.97 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O15259, Q9QY53
• Gene Symbols: NPHP1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 463-732 of human NPHP1 (NP_9970642).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG