Invitrogen™ NR3C2 Monoclonal Antibody (H10E4C9F)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1620

Catalog No. MA1620

Description

MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues. MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation. In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors. The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method.

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor (MR) and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain MR. The corresponding gene for the mineralocorticoid receptor is NR3C2.

Specifications

• Antigen: NR3C2
• Classification: Monoclonal
• Concentration: 1.4 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P08235, P22199, Q8QHI2
• Gene Symbols: NR3C2
• Immunogen: Aldosterone-3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat, Rabbit, Chicken
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Inhibition Assays, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: H10E4C9F
• Conjugate: Unconjugated
• Gene: NR3C2
• Gene Alias: aldosterone receptor; MCR; Mineralocorticoid receptor; Mineralocorticoid receptor (aldosterone receptor); mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; Mlr; MR; NR3C2; NR3C2VIT; nuclear hormone receptor; nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2; nuclear receptor subfamily 3, group C, member 2 variant 3
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100358449, 25672, 374131, 4306
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid