Invitrogen™ Human NRG1 beta-1 ELISA Kit

Description

ELISA

The Human NRG1beta-1 (Hu NRG1) ELISA quantitates Hu NRG1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NRG1. Principle of the method The Human NRG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.

Specifications

• Accession Number: Q02297
• Assay Range: 82.3 to 20,000 pg/mL
• Assay Sensitivity: 50 pg/mL
• Calibration Range: 50 pg/mL to 20000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: activation of transmembrane receptor protein tyrosine kinase activity; cardiac conduction system development; cardiac muscle cell differentiation; cardiac muscle cell myoblast differentiation; cell communication; cell migration; cell morphogenesis; cell proliferation; cellular protein complex disassembly; embryo development; endocardial cell differentiation; epidermal growth factor receptor signaling pathway; ERBB signaling pathway; Fc-epsilon receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glial cell fate commitment; innate immune response; locomotory behavior; mammary gland development; MAPK cascade; negative regulation of cardiac muscle cell apoptotic process; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of protein catabolic process; negative regulation of secretion; negative regulation of transcription, DNA-templated; nervous system development; neural crest cell development; neuron fate commitment; neurotransmitter receptor metabolic process; neurotrophin TRK receptor signaling pathway; peripheral nervous system development; phosphatidylinositol-mediated signaling; positive regulation of cardiac muscle cell proliferation; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of protein kinase B signaling; positive regulation of protein tyrosine kinase activity; positive regulation of Ras protein signal transduction; positive regulation of striated muscle cell differentiation; protein tyrosine kinase activator activity; regulation of protein heterodimerization activity; regulation of protein homodimerization activity; synapse assembly; transcription cofactor activity; transmembrane receptor protein tyrosine kinase activator activity; transmembrane receptor protein tyrosine kinase signaling pathway; ventricular cardiac muscle cell differentiation; ventricular trabecula myocardium morphogenesis; wound healing
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 3084
• Gene Symbol: ARIA,GGF,GGF2,HGL,HRG,HRG1,HRGA,MST131,MSTP131,NDF-IT2,SMDF,NRG1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Synonym: ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF-IT2, SMDF, NRG1
• Target: NRG1
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins