Invitrogen™ NSE Monoclonal Antibody (14)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA529236

Catalog No. PIMA529236

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ENO2.

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

Specifications

• Antigen: NSE
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P09104
• Gene Symbols: ENO2
• Immunogen: Recombinant Human ENO2 Protein (Met1-Leu434).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 14
• Conjugate: Unconjugated
• Gene: ENO2
• Gene Alias: 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; AI837106; D6Ertd375e; Eno; ENO2; Eno-2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); enolase 2, gamma neuronal; enolase 2, gamma, neuronal; epididymis secretory protein Li 279; gamma enolase; gamma-enolase; gamma-enolase-like; gamma-subunit of enolase; HEL-S-279; LOC100911625; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; neuron-specific enolase; NSE; RNEN3
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 2026
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid