Invitrogen™ NSE Polyclonal Antibody

Catalog No.	Quantity
PIPA588488	100 μL

Catalog No. PIPA588488 Supplier Invitrogen™ Supplier No. PA588488

Description

Rabbit Polyclonal Antibody

Immunogen sequence: VIKDKYGKDA TNVGDEGGFA PNILENSEAL ELVKEAIDKA GYTEKIVIGM DVAASEFYRD GKYDLDFKSP TDPSRYITGD QLGALYQDFV RDYPVVSIED PFDQDDWAAW SKFTANVGIQ IVGDDLTVTN PKRIERAVEE KACNCLLLKV NQIGSVTEAI QACKLAQENG WGVMVSHRSG ETEDTFIADL VVGLCTGQIK TGAPCRSERL AKYNQLMRIE EELGDEARFA GHNFRNPSVL; Positive Samples: HeLa, LO2, SH-SY5Y, U-251MG, Mouse skeletal muscle, Mouse liver, Mouse brain, Rat brain, Rat skeletal muscle; Cellular Location: Cell membrane, Cytoplasm.

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

Specifications

• Antigen: NSE
• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.42 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene: ENO2
• Gene Accession No.: P07323, P09104, P17183
• Gene Alias: 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; AI837106; D6Ertd375e; Eno; ENO2; Eno-2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); enolase 2, gamma neuronal; enolase 2, gamma, neuronal; epididymis secretory protein Li 279; gamma enolase; gamma-enolase; gamma-enolase-like; gamma-subunit of enolase; HEL-S-279; LOC100911625; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; neuron-specific enolase; NSE; RNEN3
• Gene Symbols: ENO2
• Host Species: Rabbit
• Immunogen: Recombinant protein (or fragment).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13807, 2026, 24334
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG