Invitrogen™ NSFL1C Polyclonal Antibody

Catalog No.	Quantity
PIPA5121024	100 μL

Catalog No. PIPA5121024 Supplier Invitrogen™ Supplier No. PA5121024

Description

Rabbit Polyclonal Antibody

Positive test controls include: 293T, U-251MG, Mouse kidney, Mouse brain, Rat brain, Rat kidney. The target is usually found in the following locations: Chromosome, Golgi apparatus, Golgi stack, Nucleus. Immunogen sequence: MAAERQEALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEDEEEEE GQRFYAGGSE RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF AGGGYRLGAA PEEESAYVAG EKRQHSSQDV HVVLK.

p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.

Specifications

• Antigen: NSFL1C
• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1.49 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene: Nsfl1c
• Gene Accession No.: O35987, Q9CZ44, Q9UNZ2
• Gene Alias: dJ776F14.1; Munc-18c; NSFL1 (p97) cofactor (p47); NSFL1 cofactor; NSFL1 cofactor p47; NSFL1C; p47; p97 cofactor p47; RP4-776F14.2; SHP1 homolog; Stxbp3a; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C; XY body-associated protein XY40; XY40
• Gene Symbols: Nsfl1c
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 386649, 55968, 83809
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG