nti-LOXL2, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABE48425UG	25 μg

Catalog No. ABE48425UG Supplier MilliporeSigma Supplier No. ABE48425UG

Description

Rabbit Polyclonal Antibody

nti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Lysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-1, LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A.

Specifications

• Antigen: nti-LOXL2
• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues. Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the  Institut Hospital del Mar d'Investigacions Mèdiques (IMIM) in Barcelona Spain ).
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Lysyl oxidase homolog 2;EC: 1.4.3.13;Lysyl oxidase-like protein 2;Lysyl oxidase-related protein 2;Lysyl oxidase-related protein WS9-1
• Gene Symbols: LOXL2
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of huamn Lysyl oxidase homolog 2.
• Molecular Weight of Antigen: ∽82 kDa obseerved; 86.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Affinity Purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detects Lysyl oxidase homolog 2 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.