MilliporeSigma™ Nuclear Pore Complex Proteins, Mouse, Unlabeled, Clone: 414,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1267

Catalog No. MABS1267MI

Description

Nuclear pore glycoprotein p62 (UniProt P17955; also known as 62kDa nucleoporin, Nucleoporin Nup62) is encoded by the Nup62 gene (Gene ID 65274) in rat species. The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex is located in the center of the NPC and contains the FG-repeat proteins Nup62, Nup54, and Nup58 associated with each other via conserved coiled-coil segments in a 1:1:1 stiochiometric ratio. The nuclear envelope breaks down during prometaphase. The disassembled nucleoporin subcomplexes act as critical regulators of various mitotic events to ensure proper chromosome segregation. Specifically, Nup62 is reported to localize to centrosomes/spindle poles, where it associates with the centrosomal proteins gamma-tubulin and hSAS-6. Nup62 is involved in the recruitment of critical proteins to the centrosome and its depletion results in mislocalization of several centrosomal components.

Specifications

• Antigen: Nuclear Pore Complex Proteins
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P17955
• Immunogen: Triton X-100 treated rat liver nuclei.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_075586
• Target Species: Human
• Form: Purified

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: 414
• Conjugate: Unconjugated
• Gene: Nup62
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 414 immunostained rat liver cell nuclear rim and yeast cell nuclear envelope (Aris, J.P., and Blobel, G. (1989). J. Cell Biol. 108(6):2059-2067; Davis, L.I., and Blobel, G. (1986). Cell. 45(5):699-709).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.