Nucleolin/NCL Polyclonal Antibody, Bethyl Laboratories

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-157-1456	Bethyl Laboratories, Inc IHC00083T	10 μL
50-157-1455	Bethyl Laboratories, Inc IHC00083	100 μL

Description

The recommended shelf life for this product is 1 year from date of receipt. Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan, Monkey, Gorilla

Nucleolin, which is identical to human DNA helicase IV, is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. This 100 kDa protein has three major domains: a N-terminal domain comprised of long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein, a central domain that contains four RNA binding elements, a C-terminal domain approximately 85 amino acids long that is rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis; intact 100 kDa protein is the major species in actively dividing cells, whereas the degraded forms are relativley abundant in nondividing cells. Nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Nucleolin also interacts directly with DNA topoisomerase I. It is located mainly in dense fibrillar regions of the nucleolus. Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. It interacts with APTX and contains 4 RNA recognition motif (RRM) domains.

Specifications

• Antigen: Nucleolin/NCL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Alias: C23, FLJ45706, FLJ59041
• Immunogen: Between 550 and C-term
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: NCL

• Applications: Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human, Mouse
• Gene Accession No.: P09405, P19338
• Gene ID (Entrez): 17975, 4691
• Isotype: IgG
• Purification Method: Antigen affinity chromatography
• Product Type: Antibody