Invitrogen™ NUDT16 Polyclonal Antibody

Catalog No.	Quantity
PIPA5110049	100 μL

Catalog No. PIPA5110049 Supplier Invitrogen™ Supplier No. PA5110049

Description

Rabbit Polyclonal Antibody

Immunogen sequence: GFVDTQDRSL EDGLNRELRE ELGEAAAAFR VERTDYRSSH VGSGPRVVAH FYAKRLTLEE LLAVEAGATR A.

RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596). [UniProt].

Specifications

• Antigen: NUDT16
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 1.56 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene: NUDT16
• Gene Accession No.: Q6P3D0, Q96DE0
• Gene Alias: 2310041H06Rik; 2810047L04Rik; 2900006H04Rik; AI851783; IDP phosphatase; IDPase; inosine diphosphate phosphatase; LOC100718706; m7GpppN-mRNA hydrolase; nucleoside diphosphate-linked moiety X motif 16; nudix; nudix (nucleoside diphosphate linked moiety X)-type motif 16; nudix hydrolase 16; Nudix motif 16; nudix-type motif 16; NUDT16; RGD1311387; testicular tissue protein Li 129; U8 snoRNA-binding protein H29K; U8 snoRNA-decapping enzyme
• Gene Symbols: NUDT16
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-130 of human NUDT16 (NP_689608.2).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 131870, 75686
• Target Species: Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG