NuMA Antibody (SPM300), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2452270.1MG

Catalog No. NBP24522701

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

NuMA Monoclonal specifically detects NuMA in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: NuMA
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: centrophilin stabilizes mitotic spindle in mitotic cells, nuclear mitotic apparatus protein 1, NUMA, NuMA protein, SP-H antigen, structural nuclear protein
• Host Species: Mouse
• Molecular Weight of Antigen: 228 kDa
• Quantity: 0.1 mg
• Research Discipline: Breast Cancer, Cell Biology, Cell Cycle and Replication, Cellular Markers, Mitotic Regulators
• Gene ID (Entrez): 4926
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: SPM300
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Symbols: NUMA1
• Immunogen: Colon carcinoma 174T cells
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
• Content And Storage: Store at 4C.
• Isotype: IgM κ

For Research Use Only