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Invitrogen™ NuPAGE™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well Non-distribution product as customer accommodation.

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Quantity:
10/Pk.
2/Pk.

Separation Range: 3.5 to 260 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini

NuPAGE™ Novex™ 4-12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional Tris-Glycine gels, NuPAGE™ Novex™ Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE™ Novex™ Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE™ gels for optimal results during day-to-day use. NuPAGE™ Novex™ Bis-Tris gels come in two sizes and multiple well formats. Features of NuPAGE™ Novex™ Bis-Tris gels:

  • Faster run times—get separation of your proteins in as little as 35 minutes
  • Longer shelf life—NuPAGE™ Novex™ Bis-Tris gels can be stored for at least 12 months at room temperature
  • Better protein integrity—optimized sample preparation process preserves your proteins
  • Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins

    Choose the right NuPAGE™ Novex™ Bis-Tris Gel for your protein separation
    Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE™ Novex™ Bis-Tris mini gels come in 3 polyacrylamide concentrations: 10%, 12%, and a 4–12% gradient. We also offer 8% gels in the midi-gel format. The gels can be run using NuPAGE™ MES or NuPAGE™ MOPS SDS running buffer to obtain different separation ranges. NuPAGE™ Novex™ Bis-Tris gels are based upon a Bis-Tris-HCl buffered (pH 6.4) polyacrylamide gel, and have an operating pH of about 7.0.

    Choose the right NuPAGE™ Novex™ Bis-Tris Gel for the size of your samples
    Gels come in two sizes: mini (10 cm x 10 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE™ Novex™ Bis-Tris gels also come in multiple well formats.

    Fast and convenient to use
    NuPAGE™ Novex™ Bis-Tris gels can be run in as little as 35 minutes when using the NuPAGE™ MES Running Buffer. NuPAGE™ Novex™ Bis-Tris gels have a shelf life of at least 12 months at +4–25°C. Unlike traditional Tris-Glycine gels, NuPAGE™ Novex™ Bis-Tris gels have a neutral pH environment that minimizes protein modifications. The gels also provide sharper bands, higher resolution, longer shelf-life, and higher accuracy of results.

    NuPAGE™ Novex™ Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE™ LDS Sample Buffer and NuPAGE™ Sample Reducing Agent with your samples. Use NuPAGE™ Antioxidant in the upper buffer chamber in order to maintain the reduced state of the proteins during the run and allow maximum band sharpness. We recommend using Novex™ Sharp Pre-stained Protein Standard; this protein standard contains our widest molecular weight range with 12 proteins bands ranging from 3.5–260 kDa. Get fast and sensitive staining of your protein gels with our SimplyBlue™ SafeStain.

    We also provide NuPAGE™ Novex™ Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-Glycine electrophoresis, we provide Novex™ Tris-Glycine gels .

    NuPAGE™ Novex™ Bis-Tris gels can be used with the XCell SureLock™ Mini Cell and the XCell4 SureLock™ Midi Cell gel apparatuses and are compatible with the iBlot™ Gel Transfer Device to create your western blots.
Adapters No Adapters
Recommended Applications Denaturing
Mode of Separation Molecular Weight
Well Design 1D Well
Shipping Condition Room Temperature
Sample Loading Volume up to 25 µL
Thickness 1.0 mm
Product Line Novex™, NuPAGE®
For Use With (Application) Protein Gel Electrophoresis
Formulation Gel
Length (Metric) 8 cm
Length (Metric) Gel 8 cm
Storage Requirements 4°C to 25°C
Thickness (English) 0.039 in.
Width (Metric) 8 cm
Width (Metric) Gel 8 cm
Product Type Gradient Page
Gel Size Mini
Separation Range 3.5 to 260 kd
Gel Percentage 4 to 12%
Gel Type Bis-Tris
Separation Type Denaturing
Gel Thickness 1.0 mm
For Use With (Equipment) Mini Gel Tank,XCell SureLock Mini
Separation of Protein
Wells 10-well
Quantity 2/Pk.
Content And Storage One box contains 2 gels. Store at 4–25°C. Do not freeze. Shelf life is 12 months.
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