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Invitrogen™ NuPAGE™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well
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- Faster run times—get separation of your proteins in as little as 35 minutes
- Longer shelf life—NuPAGE™ Novex™ Bis-Tris gels can be stored for at least 12 months at room temperature
- Better protein integrity—optimized sample preparation process preserves your proteins
- Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Choose the right NuPAGE™ Novex™ Bis-Tris Gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE™ Novex™ Bis-Tris mini gels come in 3 polyacrylamide concentrations: 10%, 12%, and a 4–12% gradient. We also offer 8% gels in the midi-gel format. The gels can be run using NuPAGE™ MES or NuPAGE™ MOPS SDS running buffer to obtain different separation ranges. NuPAGE™ Novex™ Bis-Tris gels are based upon a Bis-Tris-HCl buffered (pH 6.4) polyacrylamide gel, and have an operating pH of about 7.0.
Choose the right NuPAGE™ Novex™ Bis-Tris Gel for the size of your samples
Gels come in two sizes: mini (10 cm x 10 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE™ Novex™ Bis-Tris gels also come in multiple well formats.
Fast and convenient to use
NuPAGE™ Novex™ Bis-Tris gels can be run in as little as 35 minutes when using the NuPAGE™ MES Running Buffer. NuPAGE™ Novex™ Bis-Tris gels have a shelf life of at least 12 months at +4–25°C. Unlike traditional Tris-Glycine gels, NuPAGE™ Novex™ Bis-Tris gels have a neutral pH environment that minimizes protein modifications. The gels also provide sharper bands, higher resolution, longer shelf-life, and higher accuracy of results.
NuPAGE™ Novex™ Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE™ LDS Sample Buffer and NuPAGE™ Sample Reducing Agent with your samples. Use NuPAGE™ Antioxidant in the upper buffer chamber in order to maintain the reduced state of the proteins during the run and allow maximum band sharpness. We recommend using Novex™ Sharp Pre-stained Protein Standard; this protein standard contains our widest molecular weight range with 12 proteins bands ranging from 3.5–260 kDa. Get fast and sensitive staining of your protein gels with our SimplyBlue™ SafeStain.
We also provide NuPAGE™ Novex™ Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-Glycine electrophoresis, we provide Novex™ Tris-Glycine gels .
NuPAGE™ Novex™ Bis-Tris gels can be used with the XCell SureLock™ Mini Cell and the XCell4 SureLock™ Midi Cell gel apparatuses and are compatible with the iBlot™ Gel Transfer Device to create your western blots.
|Mode of Separation||Molecular Weight|
|Well Design||1D Well|
|Shipping Condition||Room Temperature|
|Sample Loading Volume||up to 25 µL|
|Product Line||Novex™, NuPAGE®|
|For Use With (Application)||Protein Gel Electrophoresis|
|Length (Metric)||8 cm|
|Length (Metric) Gel||8 cm|
|Storage Requirements||4°C to 25°C|
|Thickness (English)||0.039 in.|
|Width (Metric)||8 cm|
|Width (Metric) Gel||8 cm|
|Product Type||Gradient Page|
|Separation Range||3.5 to 260 kd|
|Gel Percentage||4 to 12%|
|Gel Thickness||1.0 mm|
|For Use With (Equipment)||Mini Gel Tank,XCell SureLock Mini|
|Content And Storage||One box contains 2 gels. Store at 4–25°C. Do not freeze. Shelf life is 12 months.|
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