MilliporeSigma™ O-GlcNAc, Mouse, Unlabeled, Clone: RL1,

Catalog No.	Quantity	Conjugate
MABS1253MI	100 μL	Unconjugated

Catalog No. MABS1253MI Supplier MilliporeSigma™ Supplier No. MABS1253

Description

Mouse Monoclonal Antibody

Posttranslational modification of proteins by -linked N-acetylglucosamine ( -GlcNAc) via the hydroxyl moieties on serine or threonine residues is termed O-linked -GlcNAc or simply O-GlcNAc. O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all animals and plants. Unlike other types of protein glycosylations, O-GlcNAc occurs exclusively within the nuclear and cytoplasmic compartments and is generally not further modified to form more elongated structures. In addition, O-GlcNAcylation is a highly dynamic and reversible process. The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues, while O-GlcNAcase catalyzes the removal/hydrolysis of O-GlcNAc from proteins. In fact, a dynamic interplay between O-GlcNAcylation and serine/threonine phosphorylation plays an important role in regulating cellular signaling. Tau and RNA polymerase II (Pol II) are two well known proteins that undergo modification by O-GlcNAcylation. In Alzheimer's diseased human brains, tau becomes extensively phosphorylated and less O-GlcNAcylated. Similarly, O-GlcNAc is removed and replaced with O-phosphate on the Poly II CTD when the elongation phase of transcription is initiated.

Specifications

• Antigen: O-GlcNAc
• Applications: Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Inhibition Assays
• Classification: Monoclonal
• Clone: RL1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse IgM in buffer containing PBS without preservatives.
• Host Species: Mouse
• Immunogen: Nuclear pore complex-lamina fraction isolated from rat liver nuclear envelopes.
• Purification Method: Purified by Ion-Exchange Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Both clone RL1 & clone RL2 (Mfr. No. MABS157) target O-linked N-acetylglucosamine (GlcNAc) modified (GlcNAcylated) proteins. GlcNAc removal by beta-N-acetylglucosaminidase treatment or galactosylation of GlcNAc on target proteins by galactosyltransferase treatment greatly reduced the immunoreactivity of clone RL1 & RL2 toward their target proteins. The binding affinity of RL1 and RL2 toward a given GlcNAcylated protein is dependent on both O-GlcNAc and the adjacent core protein structure (Snow, C.M., et al. (1987). J. Cell Biol. 104(5):1143-11560; Holt, G.D., et al. (1987). J. Cell Biol. 104(5):1157-1164).
• Target Species: Drosophila
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze
• Form: Purified
• Isotype: IgM κ

For Research Use Only. Not for use in diagnostic procedures.