Invitrogen™ Mouse OPG (TNFRSF11B) ELISA Kit

ELISA

Catalog Number	Mfr. No.	Quantity
EMTNFRSF11B	Invitrogen™ EMTNFRSF11B	96 Tests
50-274-1206	Invitrogen™ EMTNFRSF11BX5	5 x 96 Tests
50-255-0919	Invitrogen™ EMTNFRSF11BX10	10 x 96 Tests

Description

The Mouse Osteoprotegerin (OPG/TNFRSF11B) ELISA quantitates Ms OPG in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms OPG. Principle of the method The Mouse OPG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

Specifications

• Accession Number: O08712
• Assay Sensitivity: 1 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: OCIF,Opg,TR1
• Intraassay CV: <10
• Label or Dye: HRP
• Research Discipline: Apoptosis
• Storage Requirements: 2°C to 8°C
• Target: TNFRSF11B
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 1.65 to 1200 pg/mL
• Calibration Range: 1 pg/mL to 1200 pg/mL
• Conjugate: HRP
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 18383
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
• Synonym: OCIF, Opg, TR1
• Test Time: 1 hrs 20 mins