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OVA257-264 (SIINFEKL) peptide bound to H-2Kb Mouse anti-Mouse, PE-Cyanine7, Clone: eBio25-D1.16 (25-D1.16), eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$117.00 - $331.00

Specifications

Antigen OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Clone eBio25-D1.16 (25-D1.16)
Target Species Mouse
Conjugate PE-Cyanine7
Isotype IgG1, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-245-749 Non-distribution product as customer accommodation. View Documents Invitrogen
25574380
25 μg Each for $117.00
Add to cart
 
50-245-750 Non-distribution product as customer accommodation. View Documents Invitrogen
25574382
100 μg Each for $331.00
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Description

Description

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Specifications

Specifications

OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Mouse
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
eBio25-D1.16 (25-D1.16)
PE-Cyanine7
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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