Invitrogen™ Ovine IL-6 ELISA Kit

Ovine IL-6 quantitates ovine IL-6 in serum, plasma, supernatant.

Catalog Number	Mfr. No.	Quantity
ESH5RBX5	Invitrogen™ ESH5RBX5	5 x 96 Tests
ESH5RB	Invitrogen™ ESH5RB	96 Tests

Description

Ovine IL-6 quantitates ovine IL-6 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant ovine IL-6.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Specifications

• Accession Number: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; ChIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; Hybridoma growth factor; Ifnb2; IFN-beta-2; IL6; Il-6; ILg6; ILN; Interferon beta-2; Interleukin; interleukin 6; interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; Interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
• Assay Sensitivity: 0.15 pg/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Il6
• Intraassay CV: <10%
• Label or Dye: HRP
• Sample Volume: Plasma, 50 μL; Supernatant, 50 μL; Serum, 50 μL
• Target: IL-6
• Total Assay Time: 4 hr 45 min

• Assay Range: 0.15 to 25 pg/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): P29455
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min