p19ArfRat anti-Hamster, Human, Mouse, Clone: 12.A1.1, Rockland™

Catalog No.	Quantity
50-199-1223	100 μg

Catalog No. 50-199-1223 Supplier Rockland Immunochemicals Supplier No. 200501892

Description

Rat Monoclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Directed against mouse p19Arf protein. No reactivity is observed against human or hamster homologues. The epitope was putatively mapped to amino acids 54-62 of mouse p19Arf. BLAST analysis indicates that no significant sequence homology exists for this sequence with p19Arf homologues from other sources. This epitope is also found on mouse p16Ink4a protein. No additional information is available for reactivity with p19Arf protein from other sources.

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

Specifications

• Antigen: p19Arf
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 12.A1.1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: Cdkn2a
• Gene Accession No.: P42771, P51480, Q64364, Q8N726
• Gene Alias: Alternative reading frame; ARF; ARF-INK4a; CDK4I; Cdkn2a; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; Ink4a/Arf; INK4a-ARF; mitochondrial smARF; MTS1; p16; p16(INK4a); p16-INK4; P16ink4a; p16-INK4a; p19; p19ARF; Pctr1; Tumor suppressor ARF
• Gene Symbols: Cdkn2a
• Host Species: Rat
• Immunogen: This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 54-75 of mouse p19Arf protein.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100764983, 1029, 12578
• Target Species: Hamster, Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b