p21/CIP1/CDKN1A Antibody (CIP1/823), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24452700	Novus Biologicals NBP2445270.02MG	0.02 mg
NBP24452701	Novus Biologicals NBP2445270.1MG	0.1 mg
NBP24452702	Novus Biologicals NBP2445270.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

p21/CIP1/CDKN1A Monoclonal specifically detects p21/CIP1/CDKN1A in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: p21/CIP1/CDKN1A
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
• Gene Symbols: CDKN1A
• Isotype: IgG2a κ
• Purification Method: Protein A or G purified
• Test Specificity: This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

• Clone: CIP1/823
• Dilution: Western Blot 1 - 2 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Gene Accession No.: P38936
• Gene ID (Entrez): 1026
• Immunogen: Recombinant full-length human CDKN1A protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 21 kDa

For Research Use Only