Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

p21 (WAF1, Cip1) Rabbit anti-Human, Mouse, Rat, Polyclonal, eBioscience™ Non-distribution product as customer accommodation.

Rabbit Polyclonal Antibody

$58.50 - $139.00

Specifications

Antigen p21 (WAF1, Cip1)
Target Species Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-135-20 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14671563
10 μg Each for $58.50
Add to cart
 
50-135-21 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14671581
50 μg Each for $139.00
Add to cart
 
Description

Description

Description: The polyclonal antibody reacts with mouse, rat, and human p21; the antibody was raised against a peptide mapping to the carboxy terminus of human p21. The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

Applications Reported: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining. Applications Tested: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been tested by immunoblotting (WB) (1:200 starting dilution). Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.
Specifications

Specifications

p21 (WAF1, Cip1)
Unconjugated
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Primary
Polyclonal
PBS with 0.2% gelatin and 0.1% sodium azide
RUO
Human, Mouse, Rat
IgG
Polyclonal
Liquid
Antibody
4° C

For Research Use Only.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit