Invitrogen™ p23 Monoclonal Antibody (JJ3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3414

Catalog No. MA3414

Description

MA3-414 detects p23 from chicken, guinea pig, human, rat, mouse and rabbit samples. MA3-414 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 19 or 23 kDa protein representing p23 from rabbit reticulocyte lysate. MA3-414 can be used to immunoprecipitate both free and complexed p23. The MA3-414 antigen is recombinant human p23.

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Specifications

• Antigen: p23
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.05% sodium azide
• Gene Accession No.: G1TGF1, P83868, Q15185, Q90955, Q9R0Q7
• Gene Symbols: LOC100351480, Ptges3
• Immunogen: Recombinant human p23 protein.
• Regulatory Status: RUO
• Gene ID (Entrez): 100345490, 100351480, 100718334, 100859133, 10728, 362809, 56351
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, RNA Immunoprecipitation, Immunocytochemistry
• Clone: JJ3
• Conjugate: Unconjugated
• Gene: Ptges3
• Gene Alias: 5730442A20Rik; Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; LOC100351480; P23; p23 cochaperone; progesterone receptor; progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); prostaglandin-E synthase 3; Ptges; PTGES3; ptges3 {ECO:0000250; RGD1561913; sid 3177; Sid3177; Tebp; telomerase binding protein, p23; telomerase-binding protein p23; unactive progesterone receptor, 23 kD; UniProtKB:Q15185}
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Chicken, Guinea Pig
• Product Type: Antibody
• Isotype: IgG1