p27/Kip1 Antibody (DCS-72.F6 + KIP1/769), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24449700	Novus Biologicals NBP2444970.02MG	0.02 mg
NBP24449701	Novus Biologicals NBP2444970.1MG	0.1 mg
NBP24449702	Novus Biologicals NBP2444970.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: p27/Kip1
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Prostate Cancer, Tumor Suppressors
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
• Gene Symbols: CDKN1B
• Isotype: IgG
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.

• Clone: DCS-72.F6 + KIP1/769
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Monkey
• Gene Accession No.: P46527, P46527
• Gene ID (Entrez): 1027
• Immunogen: Mouse recombinant p27 protein (DCS-72.F6); Recombinant human CDKN1B protein (KIP1/769)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 25.5 kDa

For Research Use Only