p27/Kip1 Antibody (SX53G8), HRP, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234718H

Catalog No. NBP234718H

Description

p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: p27/Kip1
• Classification: Monoclonal
• Conjugate: HRP
• Gene Alias: CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG1 κ

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: SX53G8
• Dilution: Western Blot, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Symbols: CDKN1B
• Immunogen: Purified GST-p27 fusion protein of human origin (Uniprot: P46527)
• Quantity: 0.1 mL
• Research Discipline: Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Prostate Cancer, Tumor Suppressors
• Gene ID (Entrez): 1027
• Target Species: Human, Mouse, Rat, Primate
• Form: Purified

For Research Use Only