Invitrogen™ P4HTM Polyclonal Antibody

Catalog No.	Quantity
PIPA530752	100 μL

Catalog No. PIPA530752 Supplier Invitrogen™ Supplier No. PA530752

Description

Rabbit Polyclonal Antibody

Recommended positive controls: A549, H1299, HCT116. Predicted reactivity: Mouse (94%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Specifications

• Antigen: P4HTM
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene: P4HTM
• Gene Accession No.: Q9NXG6
• Gene Alias: 4933406E20Rik; AI853847; BB128974; EGLN4; FLJ20262; HIFPH4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4HTM; P4H-TM; PH 4; PH4; PH-4; PHD4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; RGD1311848; Transmembrane prolyl 4-hydroxylase
• Gene Symbols: P4HTM
• Host Species: Rabbit
• Immunogen: Recombinant fragment corresponding to a region within amino acids 99 and 382 of Human HIF Prolyl Hydroxylase 4.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 54681
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG