Invitrogen™ P4HTM Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530753

Catalog No. PIPA530753

Description

Recommended positive controls: A549, HeLa. Predicted reactivity: Mouse (84%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Specifications

• Antigen: P4HTM
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: P4HTM
• Gene Alias: 4933406E20Rik; AI853847; BB128974; EGLN4; FLJ20262; HIFPH4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4HTM; P4H-TM; PH 4; PH4; PH-4; PHD4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; RGD1311848; Transmembrane prolyl 4-hydroxylase
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54681
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.88 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9NXG6
• Gene Symbols: P4HTM
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PHD4. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG