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Invitrogen™ P4HTM Polyclonal Antibody
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Catalog No. APPA6560
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100 μg
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Catalog No. Quantity
APPA6560 100 μg
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Catalog No. APPA6560 Supplier Invitrogen™ Supplier No. PA116560
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Description

Rabbit Polyclonal Antibody

This antibody does not react with mouse samples. Suggested positive control: antigen standard for PH (transient overexpression lysate), HeLa and human glioblastoma cell lysates.

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
TRUSTED_SUSTAINABILITY

Specifications

Antigen P4HTM
Applications Immunohistochemistry (Paraffin), Immunomicroscopy, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation Phosphate, tris citrate with 0.09% sodium azide; pH 7-8
Gene P4HTM
Gene Accession No. Q8BG58, Q9NXG6
Gene Alias 4933406E20Rik; AI853847; BB128974; EGLN4; FLJ20262; HIFPH4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia inducible factor prolyl 4 hydroxylase; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4HTM; P4H-TM; PH 4; PH4; PH-4; PHD4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; RGD1311848; Transmembrane prolyl 4-hydroxylase
Gene Symbols P4HTM
Host Species Rabbit
Immunogen The epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human PHD4/HIF Prolyl Hydroxylase 4 using the numbering given in entry NP_8088081 (GeneID 54681).
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 301008, 54681, 74443
Target Species Human, Mouse, Rat
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov
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