p73 Antibody (5B429) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 56 publications

Catalog Number	Mfr. No.	Quantity
NBP224737SS	Novus Biologicals NBP224737SS	0.025 mg
NBP224737	Novus Biologicals NBP224737	0.1 mg

Description

p73 Monoclonal specifically detects p73 in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-ready.

Specifications

• Antigen: p73
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Cancer, Tumor Suppressors
• Gene Accession No.: O15350
• Gene ID (Entrez): 7161
• Immunogen: This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 5B429
• Dilution: Western Blot 2 ug/ml, Simple Western 1:25, Flow Cytometry 1 ug/mL. Use reported in scientific literature (PMID 21152443), Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 5-15 ug/ml, Immunohistochemistry-Frozen 5-15 ug/ml, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP) 1:20-1:1000, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Alias: p53-related protein, P73p53-like transcription factor, TA p73, TAp73, tumor protein p73
• Gene Symbols: TP73
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674/ IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).

For Research Use Only