pan-Actin, Mouse anti-Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast, Clone: 2A3, Millipore Sigma™

Catalog No.	Quantity
MABT133325	25 μL
MABT1333100	100 μL

Catalog No. MABT133325 Supplier MilliporeSigma Supplier No. MABT133325UL

Description

Mouse Monoclonal Antibody

Anti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.

Actin is a multi-functional cytoskeletal protein that serves as the basic building block of cytoskeletal microfilaments. Six different actin types have been reported. Skeletal muscle alpha-actin (UniProt: P68133) is encoded by the ACTA1 gene, smooth muscle alpha-actin (UniProt: P62736) by the ACTA2 gene, cytoplasmic beta-actin (UniProt: P60709) by the ACTB gene, cardiac muscle alpha-actin (UniProt: P68032) by the ACTC1 gene, cytoplasmic gamma-actin (UniProt: P63261) by the ACTG1 gene, and smooth muscle gamma-actin (UniProt: P63267) by the ACTG2 (also known as ACTA3) gene. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic beta and gamma actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. This pan-actin antibody detects all isoforms actin.

Specifications

• Antigen: pan-Actin
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 2A3
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Skeletal muscle alpha-actin;Smooth muscle alpha-actin;Cytoplasmic beta-actin;Cardiac muscle alpha-actin;Cytoplasmic gamma-actin;Smooth muscle gamma-actin
• Gene Symbols: ACTA1;ACTA2;ACTB;ACTC1;ACTG1;ACTG2
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
• Purification Method: Protein G purified
• Quantity: 25 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001092
• Test Specificity: Clone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
• Target Species: Mouse, Rat, Drosophila, Hamster, Human, Monkey, Yeast
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ