Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2476630.2MG

Catalog No. NBP247663B

Description

pan Actin Monoclonal antibody specifically detects Antigen in Human, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: pan Actin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Gene Alias: ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.AIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: HHF35 + MSA/953
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: ACTA1
• Immunogen: Recombinant human actin fragment
• Quantity: 0.2 mg
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Stem Cell Markers
• Gene ID (Entrez): 58
• Target Species: Human, Rat, Rabbit
• Form: Purified