Abnova™ Papain (Papaya)

Description

Used for Enzyme

#REF!

Specifications

• Accession Number: P00784
• For Use With (Application): Enzyme Activity
• Molecular Weight (g/mol): 23.4kDa
• Name: Papain (Papaya)
• Preparation Method: Native protein purified from Papaya Latex
• Quantity: 1 Tub
• Storage Requirements: Store at 4°C. Reconstituted with 5 ml of Earle's Balanced Salt Solution (EBSS) or equivalent yields a solution at 20 units of papain per ml in 1 mM L-cysteine with 0.5 mM EDTA. Do not freeze the reconstituted solution.
• Regulatory Status: RUO
• Common Name: PAPAIN
• Assays: Papain Assay; Cell isolation (more gentle than other proteases); Protein structural studies; Peptide mapping; Red cell surface modification for antibody screening or identification; Fab preparation from IgG and IgM antibodies; Solubilization of integral membrane proteins; Production of glycopeptides from purified proteoglycans; enzymatic wound debridement.
• Biological Activity: ≥100 units per vial. One Unit hydrolyzes one micromole of benzoyl-L-arginine ethyl ester per minute at 25°C, pH 6.2, after activation in a solution containing 1.1mM EDTA, 0.067 mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes.
• Species: Plants
• Protein Tag: None
• Expression System: Native protein purified from Papaya Latex
• Form: Lyophilized