Papain Polyclonal Antibody, Rockland™

Goat Polyclonal Antibody

Supplier:  Rockland Immunochemicals 2001174S

Catalog No. 50-199-0448

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined.

Papain is a protease derived from the papaya fruit (Carica papaya). It hydrolyses peptides with broad specificity, but shows a preference for large hydrophobic amino acids in the P2 position. It is synthesised as an inactive 40 kDa precursor containing a 107 residue N terminal pro region and a 26 residue signal peptide.

Specifications

• Antigen: Papain
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: Carica papaya
• Immunogen: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
• Regulatory Status: RUO
• Target Species: Plant
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Host Species: Goat
• Quantity: 25 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized